Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B [Illumina]

ABSTRACT: The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B re-assembles onto the male genome during protamine-to

ORGANISM(S): Mus musculus

SUBMITTER: Sophie Rousseaux 

PROVIDER: E-GEOD-46136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress