Production of pyomelanin in the Vibrio campbellii hmgA mutant results in the repression of quorum sensing, bioluminescence and virulence

ABSTRACT: Vibrio campbellii BAA-1116 was used as model organism from the Harveyi clade to understand how melanization affected cellular phenotype, metabolism and virulence. An in-frame deletion of the homogentisate-1,2-dioxygenase (hmgA) gene resulted in the overproduction of a pigment in cell culture supernatants and cellular membranes that was identified as pyomelanin. Unlike previous demonstrations in Vibrio cholerae, Burkholderia cepacia and Pseudomonas aeruginosa, the pigmented V. campbellii mutant did not show increased UV or hydrogen peroxide resistance and was found to be ~2.7 times less virulent then the wild type strain in Penaeus monodon shrimp virulence assays. Microarray-based transcriptomic analyses revealed that the deletion of the hmgA gene and subsequent pyomelanin production negati

ORGANISM(S): Vibrio campbellii

SUBMITTER: Baochuan Lin 

PROVIDER: E-GEOD-46223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress