Overcoming the stochastic phase of iPS cells reprogramming by a pulse of C/EBPα [array]

ABSTRACT: Somatic cell reprogramming into pluripotent stem cells induced by Oct4, Sox2, Klf4 and Myc (OSKM) occurs at low frequencies and with a considerable delay involving a stochastic phase. In contrast, transdifferentiation of B cells into macrophages induced by C/EBPα is fully efficient and initiated almost immediately. We now discovered that a pulse of C/EBPα in B cell precursors followed by OSKM expression dramatically enhances reprogramming to pluripotency, overcoming the stochastic phase. The cells simultaneously activate mesenchymal-epithelial transition and pluripotency genes to high levels within 2 days, correlating with promoter demethylation and accessibility to Oct4 binding. The conditioning effect of C/EBPα is shared with C/EBPβ but not with transcription factors that induce eryt

ORGANISM(S): Mus musculus

SUBMITTER: Bruno Di Stefano 

PROVIDER: E-GEOD-46321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress