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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression from Staphylococcus aureus strain USA300-lac during low and 2M NaCl exposure

ABSTRACT: S. aureus has the propensity to survive a range of NaCl challenge conditions We used commercially available Affymetrix S. aureus GeneChips (part number 900514) to compare the gene expression properties of wild type cells during growth at no or high (2M) NaCl. S. aureus strain USA300-lac cells were grown to late exponential phase growth in the absence or presence of 2M NaCl, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of high NaCl.

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300

SUBMITTER: Paul Dunman

PROVIDER: E-GEOD-46383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression from Staphylococcus aureus strain USA300-lac during low and 2M NaCl exposure

Project description:S. aureus has the propensity to survive a range of NaCl challenge conditions We used commercially available Affymetrix S. aureus GeneChips (part number 900514) to compare the gene expression properties of wild type cells during growth at no or high (2M) NaCl.

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Staphylococcus aureus subsp. aureus USA300

Project description:Expression from Staphylococcus aureus strain USA300-lac during low and 2M NaCl exposure

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Expression analysis of Staphylococcus aureus USA300 LAC in response to vehicle and benzbromarone.

Project description:Compilation fo whole genome gene expression changes in Staphylococcus aureus USA300 LAC cultures grown in the presence of vehicle or the anti-gout drug benzbromarone. The drug was intially screened as effective against the agr quorum sensing system in Staphylococcus aureus AH1677. A microarray study using total RNA harvested from three cultures of Staphylococcus aureus USA300 LAC plus vehicle control and three cultures of Staphylococcus aureus USA300 LAC plus 12 uM benzbromarone.

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