Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma)

ABSTRACT: In this study we show that lysyl oxidase (LOX), an enzyme involved in maintaining structural integrity of the extracellular matrix, is expressed at low levels in Ewing sarcoma cells and primary tumors and is downregulated by the EWS/FLI1 oncoprotein characteristic of these tumors. Using a doxycycline inducible system to restore LOX expression in an Ewing sarcoma derived cell line, we show that LOX displays tumor suppressor activities. Interestingly, we show that the tumor suppressor activity resides in the propeptide domain of LOX (LOX-PP), an N-terminal domain produced by proteolytic cleavage during the physiological processing of LOX. Finally, we show that LOX-PP inhibits ERK/MAPK signalling pathway, and that many pathways involved in cell cycle progression were significant deregulated b

ORGANISM(S): Homo sapiens

SUBMITTER: Javier Alonso 

PROVIDER: E-GEOD-46407 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress