High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI

ABSTRACT: In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method. According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation. Extracting the MspJI digested fragments, constructing sequencing library according to the Illumina protocol and sequencing with Illumina HiSeq2000. Repeatability and reproducibility studies were performed between two samples from the same individual. Specificity

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yudong Xia 

PROVIDER: E-GEOD-46428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress