Selective enhancement and repression of glucocorticoid receptor signaling by coregulator Hic-5

ABSTRACT: The glucocorticoid receptor (GR) recruits many coregulators via the well characterized AF2 interaction surface in the GR ligand binding domain, but LIM domain coregulator Hic-5 binds to the relatively uncharacterized tau2 activation domain in the hinge region of GR. Requirement of Hic-5 for glucocorticoid-regulated gene expression in U2OS osteosarcoma cells was defined by Hic-5 depletion and global gene expression analysis. Hic-5 depletion had selective and dramatic effects, positive and negative, on both activation and repression of GR target genes. For some hormone-induced genes, Hic-5 facilitated recruitment of the Mediator complex and RNA polymerase II. In contrast, many genes were not regulated by hormone until Hic-5 was depleted. On these genes Hic-5 acted at a very early step of the

ORGANISM(S): Homo sapiens

SUBMITTER: Dai-Ying Wu 

PROVIDER: E-GEOD-46448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress