Transcription profiling of brown adipose tissue in a glycerol kinase (Gyk) knockout mouse model to define GyKs role in insulin resistance

ABSTRACT: Glycerol kinase (GK) is at the interface of fat and carbohydrate metabolism and has been implicated in insulin resistance and type 2 diabetes mellitus (T2DM). To define GK's role in insulin resistance, we examined gene expression in brown adipose tissue in a glycerol kinase (Gyk) knockout (KO) mouse model using microarray analysis. Global gene expression profiles of KO mice were distinct from wild type (WT) with 668 genes that were differentially expressed. These included genes involved in lipid metabolism, carbohydrate metabolism, insulin signaling, and insulin resistance. Real-Time (RT) PCR analysis confirmed the differential expression of selected genes involved in lipid and carbohydrate metabolism. PathwayAssist analysis confirmed direct and indirect connections between GK and genes in

ORGANISM(S): Mus musculus

SUBMITTER: Lola Rahib 

PROVIDER: E-GEOD-4656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress