D-Glutamate Utilization in Pseudomonas aeruginosa PAO1

ABSTRACT: D-Glutamate (D-Glu), an essential component of peptidoglycans, can be utilized as carbon and nitrogen source by Pseudomonas aeruginosa. DNA microarrays were employed to identify genes involved in D-Glu catabolism. Through gene knockout and growth phenotype analysis, the divergent dguR-dguABC (D-glutamate utilization) gene cluster was shown to participate in D-Glu catabolism and regulation. The dguA gene encodes a FAD-dependent D-amino acid dehydrogenase with D-Glu as its preferred substrate, and its promoter was specifically induced by exogenous D-Glu and D-Gln. The function of DguR as transcriptional activator of the dguABC operon was demonstrated by promoter activity measurements in vivo and by mobility shift assays with purified His-tagged DguR in vitro. Although the DNA-binding activit

ORGANISM(S): Pseudomonas aeruginosa PAO1

SUBMITTER: CHun-Kai Yang 

PROVIDER: E-GEOD-46603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress