A transcriptome-wide atlas of RNP compositions reveals diverse classes of mRNAs and lncRNAs

ABSTRACT: Aim: To determine how different classes of transcript (e.g. lncRNAs and mRNAs) are defined in the cell. Approach: We determined the transcriptome-wide targets of key RNA packaging, maturation, export and turnover factors. We used the CRAC technique, whereby RNA:protein interactions are fixed by UV irradiation of yeast cultures, and RNA:protein complexes obtained via a stringent multi-step purification. A mild RNase treatment fragments the bound RNAs, which are then used as templates for RT-PCR, prior to sequencing. This approach enabled us to compare the maturation and turnover pathways of mRNAs and lncRNAs. Results: Our data reveal that mRNA and lncRNA maturation pathways diverge prior to nuclear export, and 3' end processing emerges as a key step in determining transcript fate. Our ana

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Alex Tuck 

PROVIDER: E-GEOD-46742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress