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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiles of NCTC11168 grown with or without 25mM fucose

ABSTRACT: Strain specific growth of C. jejuni on fucose has been linked to a plasticity region of the chromosome (PR2) and confers a competitive advantage during intestinal colonization. Growth on fucose induces gene expression of PR2 genes, but the regulatory mechanism of the structural genes involved with fucose utilization is unknown. Additionally, the mechanism of fucose dissimilation by C. jejuni is not known since no fucose catabolism homologs are found in the C. jejuni genome. Transcriptional profiles of C. jejuni grown with and without fucose may provide insight in to the genes that are necessary for fucose utilization. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. Each sample represents one competitive hybridization: sham-treated NCTC 11168 v.s. 25mM fucose treated NCTC 11168. There were four biological replicates of each sample with a dye swap introduced in alternating replicates. Samples were independently grown, treated and harvested.

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Wayne Muraoka

PROVIDER: E-GEOD-46752 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiles of wild-type NCTC11168 and Cj0480c mutant grown without fucose

Project description:Strain specific growth of C. jejuni on fucose has been linked to a plasticity region of the chromosome (PR2) and confers a competitive advantage during intestinal colonization. Growth on fucose induces gene expression of PR2 genes, but the regulatory mechanism of the structural genes involved with fucose utilization is unknown. A mutant was constructed to examine the role of Cj0480c, a putative IclR-type transcriptional regulator, on PR2 gene expression. Transcriptional profiles of wild-type C. jejuni and the Cj0480c mutant strain grown without fucose may provide insight in to the extent of the fucose regulon and genes that are necessary for fucose utilization. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. Each sample represents one competitive hybridization: wild-type NCTC 11168 v.s. Cj0480c isogenic mutant. There were four biological replicates of each sample with a dye swap introduced in alternating replicates. Samples were independently grown, treated and harvested.

2014-01-01 | E-GEOD-46808 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 4mg/L erythromycin treatment

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2013-10-10 | E-GEOD-43023 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 0.125mg/L erythromycin treatment

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2013-10-10 | E-GEOD-43024 | biostudies-arrayexpress

Transcriptional profiles of NCTC11168 grown with or without 25mM fucose

2014-01-01 | GSE46752 | GEO

Transcriptional profiles of wild-type NCTC11168 and Cj0480c mutant grown without fucose

2014-01-01 | GSE46808 | GEO

CGH Analysis of C. jejuni isolates

Project description:We performed microarray CGH analysis of 104 neuropathogenic and enteritis only stains of C. jejuni. Keywords: Individual hybridizations, CGH We measured signal intensity for tester strain versus control strain Cj NCTC 11168 for 104 isolates of C. jejuni.

2007-09-30 | E-GEOD-3579 | biostudies-arrayexpress

CGH analysis of Campylobacter jejuni isolates previously analyzed by Multi-Locus Sequence Typing

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2008-04-21 | E-GEOD-9919 | biostudies-arrayexpress

Global functional impacts of Campylobacter jejuni stress induced by exposure to deoxycholate

Project description:Comparison of the Campylobacter jejuni NCTC 11168 proteome in MH media in the presence and absence of Deoxycholate

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Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- α media

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type versus LuxS01 mutant strain grown in a minimal culture medium (MEM- α ) versus complex culture medium (MHB).

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The role of the Cj0440c gene in erythromycin-resistant (Eryr) Campylobacter jejuni

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