Transcription profiling of rat peritoneal mesotheliomas following treatment with o-nitrotoluene or bromochloracetic acid

ABSTRACT: This study was performed to characterize the gene expression profile of rat peritoneal mesothelioma (RPM) formation following treatment of Fischer 344 rats with o-nitrotoluene (o-NT) or bromochloracetic acid (BCA). Experiment Overall Design: Four mesotheliomas from o-NT-treated rats and four mesotheliomas from BCA-treated rats were selected for microarray analysis. At necrosy, the tumors were collected, minced and flash frozen in liquid nitrogen. A non-transformed mesothelial cell line, Fred-PE (passage eight), was used as a source of reference RNA. Total RNAs from tumor tissue and Fred-PE were isolated with the RNeasy kit and 500 ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol. Fo

ORGANISM(S): Rattus norvegicus

DISEASE(S): normal

SUBMITTER: NIEHS Microarray Group 

PROVIDER: E-GEOD-4682 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress