DNA replication and DSB factor binding in the meiotic S-phase checkpoint in budding yeast

ABSTRACT: During gamete formation, crossover recombination must occur on replicated DNA to ensure proper chromosome segregation in the first meiotic division. We identified a Mec1/ATR-dependent replication checkpoint in budding yeast that prevented the earliest stage of recombination, the programmed induction of DNA double-strand breaks (DSBs), when pre-meiotic DNA replication was delayed. The checkpoint suppressed DSBs through three complementary mechanisms: inhibition of Mer2 phosphorylation by Dbf4-dependent Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes. Importantly, such DSBs frequently failed to be repaired and impeded further DNA synthesis, leading t

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andreas Hochwagen 

PROVIDER: E-GEOD-46841 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress