Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data for resting and activated B and T cells

ABSTRACT: The IgH locus encodes for part of the antibody exposed by B cells and is important for the immune system. In B cells, one allele produces protein, the other must remain silenced. It was proposed that both alleles reside in different nuclear compartments and that this is important to maintain mono-allelic productivity. Here we challenge this concept. We provide detailed genome-wide contact maps, which show that IgH adopts different nuclear locations in immune versus other cells but also demonstrate that in B cells both alleles reside in the same environment. Nuclear positioning is therefore not important to maintain allelic exclusion. We did a gene expression analysis on resting (Day 0) and in vitro activated (Day 4) B and T cells in triplicate Splenic B cells and T cells were separately isolated from spleen, using magnetic activated cell sorting, and activated in vitro for 4 days

ORGANISM(S): Mus musculus

SUBMITTER: sjoerd holwerda 

PROVIDER: E-GEOD-46865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Allelic exclusion of the immunoglobulin heavy chain locus is independent of its nuclear localization in mature B cells.

Holwerda Sjoerd J B SJ   van de Werken Harmen J G HJ   Ribeiro de Almeida Claudia C   Bergen Ingrid M IM   de Bruijn Marjolein J W MJ   Verstegen Marjon J A M MJ   Simonis Marieke M   Splinter Erik E   Wijchers Patrick J PJ   Hendriks Rudi W RW   de Laat Wouter W  

Nucleic acids research 20130607 14

In developing B cells, the immunoglobulin heavy chain (IgH) locus is thought to move from repressive to permissive chromatin compartments to facilitate its scheduled rearrangement. In mature B cells, maintenance of allelic exclusion has been proposed to involve recruitment of the non-productive IgH allele to pericentromeric heterochromatin. Here, we used an allele-specific chromosome conformation capture combined with sequencing (4C-seq) approach to unambigously follow the individual IgH alleles  ...[more]

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