Microarray-based gene expression profiling of peripheral blood mononuclear cells in dairy cows with experimental hypocalcaemia and milk fever

ABSTRACT: Peripheral blood mononuclear cells (PBMC) from cows with experimentally induced hypocalcaemia or spontaneous milk fever were subject of an oligo-microarray analysis following quantitative real-time reverse transcription PCR (q-PCR). In experimental hypocalcaemia induced by 10 % Na2EDTA infusion (n = 4), the microarray revealed that 32 genes were significantly up- or down-regulated compared to control treatment by 11 % CaEDTA infusion (n = 4). In milk fever cases (n = 8), the microarray demonstrated that 98 genes were expressed differentially higher or lower compared to healthy parturient cows (n = 5). From both data, five genes were selected to be strongly related to both experimental hypocalcaemia and milk fever. Additionally, another gene was judged to be specific for milk fever independ

ORGANISM(S): Bos taurus

SUBMITTER: Kouya Sasaki 

PROVIDER: E-GEOD-46901 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress