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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional response of Schizosaccharomyces pombe to 5-Fluorouracil (5FU)

ABSTRACT: We use high-density tiling DNA microarray technology to obtain the specific transcriptome-wide response induced by 5-Fluorouracil (5FU) in the eukaryotic model Schizosaccharomyces pombe. Schizosaccharomyces pombe cultures with 5FU added to a final concentration of 500 M-NM-

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Luis Quintales

PROVIDER: E-GEOD-46919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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New insights into the RNA-based mechanism of action of the anticancer drug 5'-fluorouracil in eukaryotic cells.

Mojardín Laura L Botet Javier J Quintales Luis L Moreno Sergio S Salas Margarita M

PloS one 20131101 11

5-Fluorouracil (5FU) is a chemotherapeutic drug widely used in treating a range of advanced, solid tumours and, in particular, colorectal cancer. Here, we used high-density tiling DNA microarray technology to obtain the specific transcriptome-wide response induced by 5FU in the eukaryotic model Schizosaccharomyces pombe. This approach combined with real-time quantitative PCR analysis allowed us to detect splicing defects of a significant number of intron-containing mRNA, in addition to identify ...[more]

PMID: 24223771

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Clustered regulatory signals at nucleosome-depleted regions punctuate a constant nucleosomal landscape in Schizosaccharomyces pombe

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2013-11-25 | E-GEOD-41770 | biostudies-arrayexpress

Transcriptional response of Schizosaccharomyces pombe to 5-Fluorouracil (5FU)

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Project description:Transcriptional response of Schizosaccharomyces pombe to 5-Fluorouracil (5FU)

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Analysis of DNA strand-specific differential expression with high density tiling microarrays

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