ABSTRACT: au13-01_pi-plc What are the genes that are regulated by basal PI-PLC? Arabidopsis thaliana cells in suspension (5-days old). Cultivated in environment (middle) Gamborg+2,4-D. Added inhibitors 4 hr before extraction of the RNAs. We try to determine the role of phospholipases C ( PI-PLC) in the control of the basal expression of the genes of suspension of Arabidopsis thaliana. A first experience in which we used the edelfosine as inhibitor allowed us to identify numerous genes the basal expression of which is altered by this inhibition. We want to confirm these results with another inhibitor. 4 dye-swap - treated vs untreated comparison - treatment variations
Project description:rs09-04_plc_2 - edelfosine and wortmannin effect at 22°c and 4°c - Is there an overlap between PI4-kinase and phospholipase C signaling in cold response ? - Experiments were carried out with Columbia suspension cells. Cells were treated or not with edelfosine (PLC inhibitor) or wortmannin (PI4-kinase inhibitor). These agents were added 15 min before cold stress. ARN were extracted 4h after stress. Controls were made at 22°C. Keywords: treated vs untreated comparison 11 dye-swap - CATMA arrays
Project description:au13-06_fit - bhlh39 overexpression - Analyse the responses to bHLH39 overexpression. bHLH39 is one of the transcription factors regulating iron homeostasis responses. - Six day-old seedlings (wild type and bHLH39 overexpressor) were grown on Hoagland medium. Full seedlings were harvested for analysis. 21 dye-swap - normal vs transgenic comparaison
Project description:cea12-02_chlorosap - comparison after 4 and 52h - To observe the effects of guanosine penta/tetraphosphate on gene expression - Plants of four different genotypes (43A10.6, 44B1.3, H1 P3.2 and Col-0) were grown for ten days on MS/2 plates with 1% sucrose under 80µE light in long-day conditions (16 hours of light, 8 hours of dark). Plants were treated at 10:30 in the morning (Time 0; 10:30 is 3 and a half hours after the onset of the light period) by flooding with 30uM dexamethasone (in aqueous solution with 0,3 % DMSO and 0,033 % Silwet L-77) for 5 minutes . Samples were taken at 4 hrs and 52 hrs after T0. Aerial parts of the seedlings were rapidly harvested and frozen in liquid nitrogen. 9 dye-swap - normal vs transgenic comparaison,time course
Project description:ra12-06_myb118; myb118 mutation What are the genes over expressed or down regulated in seeds in response to a myb118 mutation? Plants of the different genotypes (wild-type, myb118 mutant, myb118 antisense lines named 0E3 and T16) were grown in a green house, their flower were tagged and seeds were harvested 10 days after anthesis. Seeds were extracted from the siliques, stored at -80°C prior to RNA extraction. 6 dye-swap - normal vs transgenic comparaison
Project description:Identification of genes induced with the cold via inhibition of the phospholipase C - To answer this question we chose a pharmacological approach. We decided to use molecules that are going to inhibit PLC. U73343 is an inactive homolog of U73122 (inhibitor of the phospholipase C pathway). Keywords: treated vs untreated comparison 3 dye-swap - CATMA arrays