Expressed dsRNA provokes selective sequence-independent PKR response in transient co-transfection experiments

ABSTRACT: In mammals, double-stranded RNA (dsRNA) plays roles in sequence-specific RNA interference, sequence-independent interferon response, and RNA editing by adenosine deaminases. We have previously shown that long hairpin dsRNA expression in cultured cells does not activate the interferon response, it is poorly processed into siRNAs, and it is partially edited. Here, we demonstrate that dsRNA expressed from transiently transfected plasmids strongly inhibits expression of co-transfected reporter plasmids but not expression of endogenous genes or reporters stably integrated in the genome. The inhibition is concentration-dependent and independent of a cell type, transfection method, or dsRNA sequence. The inhibition occurs at the level of translation and is mediated by protein kinase R (PKR). PKR

ORGANISM(S): Homo sapiens

SUBMITTER: Radek Malik 

PROVIDER: E-GEOD-46959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress