Chemical map of S. pombe reveals species-specific features in nucleosome positioning

ABSTRACT: Knowing the exact positions of nucleosomes not only advances our understanding of their role in gene regulation, but also the mechanisms that underlie between-species variation in chromatin structure. We have generated a chemical map of nucleosomes in vivo in Schizosaccharomyces pombe at base pair resolution. This new map reveals that S.pombe genome shares a similar periodic linker length distribution with Saccharomyces cerevisiae, but with major distinctions in nucleosomal/linker DNA sequence features. In S.pombe, A/T rich sequences are enriched in the nucleosome core region, particularly +/-20 bp of dyad, while they are disfavored in S.cerevisiae nucleosomes. The poly (dA-dT) tracts only slightly affect the nucleosome occupancy in S.pombe; and they possess preferential rotational positio

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Ji-Ping Wang 

PROVIDER: E-GEOD-46975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress