The pre-ovulatory LH surge elicits different effects on the granulosa- and theca-specific expression profiles in bovine follicles

ABSTRACT: The molecular mechanisms that regulate the pivotal transformation processes observed in the follicular wall following the pre-ovulatory LH-surge, are still not established, particularly for cells of the thecal layer. To elucidate thecal and granulosa cell type-specific biological functions and signaling pathways, large dominant bovine follicles were collected before and 21 hrs after an exogenous GnRH induced LH surge. Because LH receptor density varies within the granulosa cell populations, antral granulosa (aGC; those aspirated by follicular puncture) and membrane associated granulosa (mGC; those scraped from the follicular wall) were compared to thecal cell expression profiles determined by mRNA microarrays. Thecal cell gene expression was less affected in the peri-ovulatory follicle whe

ORGANISM(S): Bos taurus

SUBMITTER: Lane Christenson 

PROVIDER: E-GEOD-46996 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress