Measuring expression from randomly-designed DNA in S. cerevisiae

ABSTRACT: Here, we integrated essentially random synthetic DNA sequences into the yeast genome (HO locus) and measured the expression via custom Agilent tiling array. The purpose of this was to compare the predictions of where transcription would occur output by our computational model to the expression observed in the cell. The four constructs (A1B1, A1B2, A2B1, A2B2) were integrated into S. cerevisiae, cells were grown and from these both gDNA and mRNA were isolated. G-coupled dyes were used to label samples with Cy3 for mRNA and Cy5 for gDNA. Samples were sheared/hydrolyzed to an average size of about 250bp, hybridized to the array, and quantified. The GFF files include features of the region tageted for tiling array analysis in gff format (https://genome.ucsc.edu/FAQ/FAQformat.html#format3).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Carl de Boer 

PROVIDER: E-GEOD-47004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress