Gene Expression Profiling in Polycythemia Vera (PV)

ABSTRACT: To define the molecular abnormalities at the stem cell level in polycythemia vera (PV), we examined global gene expression in circulating CD34+ cells from 19 JAK2 V617F-positive PV patients and 6 normal individuals using Affymetrix oligonucleotide microarray technology. We observed that CD34+ cell gene expression not only differed between the PV patients and the normal controls but also between men and women PV patients. Based on these gender-specific differences in gene expression, we were able to identify 102 genes differentially regulated concordantly by both men and women, which likely represent a core set of genes whose dysregulation is involved in the pathogenesis of PV. Gene expression was verified by Q-PCR of patient CD34+ cell RNA. Using the 102 gene set and unsupervised hierarchi

ORGANISM(S): Homo sapiens

SUBMITTER: Jerry Spivak 

PROVIDER: E-GEOD-47018 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress