Response and adaptation to growth with low glucose concentration

ABSTRACT: The faecal indicator bacterium Escherichia coli K12 was used to study the cellular events that take place at the transcription level using the microarray technology during short-term (physiological) and long-term (genetic) adaptation to slow growth under limited nutrient supply. Short-term and long-term adaptation were assessed by comparing the mRNA levels isolated after 40 or 500 hours of glucose-limited continuous culture at a dilution rate of 0.3 h-1 with those from batch culture with glucose excess. Wild-type Escherichia coli K12 MG 1655 (ATCC 700926) was grown at 37 °C in mineral medium. Growth was followed by measuring OD546 in regular intervals to confirm that cells were growing at �max (0.63 ± 0.02 h-1). A sample for transcriptome analysis was taken from the batch exponential

ORGANISM(S): Escherichia coli

SUBMITTER: Alessandro Franchini 

PROVIDER: E-GEOD-4706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress