Dissecting the specific role of Oct4 during the early stage of reprogramming

ABSTRACT: Differentiated cells can be reprogrammed into induced pluripotent stem cells (iPSCs) following the forced overexpression of a specific combination of transcription factors, of which Oct4 is essential. To better understand the mechanism underlying iPSC reprogramming, we investigated the immediate transcriptional response to ectopic Oct4 expression in somatic cells. To this end, we derived different somatic cell types from Oct4-inducible transgenic mice and induced Oct4 expression for different time periods. Using microarray analysis, we have identified early transcriptional Oct4 targets, including genes not previously known to interact with Oct4. Comparison of the sets of Oct4-regulated genes revealed pronounced differences among the individual cell types. Furthermore, a notable up-regulat

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-47061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress