Identification of novel imprinted differentially methylated regions by global analysis of human parthenogenetic induced pluripotent stem cells

ABSTRACT: Parental imprinting is an epigenetic phenomenon by which genes are expressed in a monoallelic fashion, according to their parent-of-origin. DNA methylation is considered the hallmark mechanism regulating parental imprinting. To identify imprinted differentially methylated regions (DMRs), we compared the DNA methylation status between multiple normal and parthenogenetic human pluripotent stem cells (PSCs) by performing reduced representation bisulfite sequencing. Our analysis identified over twenty previously unknown imprinted DMRs in addition to the known DMRs. These include DMRs in loci associated with human disorders, and a class of intergenic DMRs that do not seem to be related to gene expression. Furthermore, the study showed some DMRs to be unstable, liable to differentiation or repro

ORGANISM(S): Homo sapiens

SUBMITTER: Rahul Karnik 

PROVIDER: E-GEOD-47088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress