Improved genome-wide mapping of uncapped and cleaved transcripts in eukaryotes—GMUCT 2.0

ABSTRACT: The advent of high-throughput sequencing has led to an explosion of studies into the diversity, expression, processing, and lifespan of RNAs. Recently, three different high-throughput sequencing-based methods have been developed to specifically study RNAs that are in the process of being degraded. All three methods—genome-wide mapping of uncapped and cleaved transcripts (GMUCT), parallel analysis of RNA ends (PARE), and degradome sequencing—take advantage of the fact that Illumina sequencing libraries use T4 RNA ligase 1 to ligate an adapter to the 5’ end of RNAs that have a free 5’-monophosphate. This condition for T4 RNA ligase 1 substrates means that mature mRNAs are not substrates of the enzyme because they have a 5’-cap. As a result, these sequencing libraries are specifically mad

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Nathan Berkowitz 

PROVIDER: E-GEOD-47121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress