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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from visceral mesothelium (omentum) and parietal meosthelium

ABSTRACT: Mesothelia, which cover all coelomic organs and body cavities in vertebrates, perform diverse functions in embryonic and adult life. Yet, mesothelia are traditionally viewed as simple, uniform epithelia. We used microarrays to demonstrate distinct differences between visceral and parietal mesothelia, the most basic subdivision of this tissue type, in terms of gene expression. Visceral mesothelium was isolated from the omentum of adult Wt1-cre;Rosa26ReYFP mice. In parallel, parietal mesothelium was isolated by teasing the mesothelium from the underlying skeletal muscle of the body wall of these animals. RNA was then extracted from these native mesothelia samples and used for hybridization in a microarray experiment.

ORGANISM(S): Mus musculus

SUBMITTER: David Bader

PROVIDER: E-GEOD-47161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from visceral mesothelium (omentum) and parietal meosthelium

Project description:Mesothelia, which cover all coelomic organs and body cavities in vertebrates, perform diverse functions in embryonic and adult life. Yet, mesothelia are traditionally viewed as simple, uniform epithelia. We used microarrays to demonstrate distinct differences between visceral and parietal mesothelia, the most basic subdivision of this tissue type, in terms of gene expression.

2013-05-22 | GSE47161 | GEO

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Project description:Expression data from visceral mesothelium (omentum) and parietal meosthelium

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Project description:The gene expression profiles of murine visceral and parietal mesothelia were analyzed by microarray. Visceral and parietal mesothelia were isolated from the same adult Wt1-cre;Rosa26ReYFP reporter mice and analyzed. Whole tracscriptome expression analysis of total RNA was performed using Affymetrix mouse Gene 1.0 ST arrays.

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