Identification of several potential chromatin binding sites of HOXB7 and its downstream target genes in breast cancer

ABSTRACT: We report the chromatin binding sites of HOXB7 transcription factor in BT-474 breast cancer cell line using ChIP-sequencing. We validated the chromatin binding sites in BT-474, MDA-MB-361, MCF7 and T-47D breast cancer cell lines using ChIP-qPCR. The ChIP experiments have been performed using HOXB7 antibody and IgG non-specific antibody as a negative control. The direct downstream target genes of HOXB7 were identified by analyzing the expression of genes located nearby HOXB7 binding sites in HOXB7 knockdown versus control cells using qRT-PCR. Examination of chromatin binding sites of HOXB7 in BT-474 breast cancer cell line using ChIP-seq. Four parallel IgG samples were sequenced, merged together and used as a control data set. Two parallel HOXB7 ChIP samples were sequenced and merged for ea

ORGANISM(S): Homo sapiens

SUBMITTER: Henna Heinonen 

PROVIDER: E-GEOD-47164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress