Methylomic analysis identifies epigenetically silenced genes and implies an activation of β-catenin signal in cervical cancer

ABSTRACT: Using the pools of DNA from squamous cervical carcinomas (SCC) and DNA from normal scraping cells discovered the abnormal changes of genomic wide methylation by methylation BeadChip. The 61 genes were selected to validate by quantitative methylation specific PCR (qMSP) and bisulfite pyrosequencing in further processes. A CpG islands methylator phenotype (CIMP) was confirmed at 14 candidates in an independent set contained a spectrum of scraping cells form abnormal cervical lesions. In addition, we found among of 7 genes were announced to imply to potentially biological functions of β-catenin signal. A pool DNA collected from equal amount of DNA of 38 SCC, and the other pool collected from equal amount of DNA of 19 normal cervical scraping cells. Based on 40 percent of different methylatio

ORGANISM(S): Homo sapiens

SUBMITTER: Hung-Cheng Lai 

PROVIDER: E-GEOD-47351 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress