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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Xenorhabdus nematophila FliZ regulon identification by RNA-Seq

ABSTRACT: A fliZ mutant in the entomopathogenic bacterium X. nematophila is attenuated in virulence in the insect. The goal of this study is to compare transcriptomes of the fliZ mutant and wild type strain to identify the FliZ regulon. Two biological replicates of total RNA from exponential cultures of WT strain and fliZ mutant were analysed by deep sequencing, using Illumina HiSeq 2000.

ORGANISM(S): Xenorhabdus nematophila

SUBMITTER: Sophie Gaudriault

PROVIDER: E-GEOD-47365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Xenorhabdus nematophila FliZ regulon identification by RNA-Seq

Project description:A fliZ mutant in the entomopathogenic bacterium X. nematophila is attenuated in virulence in the insect. The goal of this study is to compare transcriptomes of the fliZ mutant and wild type strain to identify the FliZ regulon.

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Genome-wide transcriptional responses of two metal-tolerant symbiotic Mesorhizobium isolates to zinc and cadmium exposure

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Project description:Transcriptional profiling of C. elegans young adult worms exposed to pathogen Xenorhabdus nematophila for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode Pristionchus pacificus to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Bacillus thuringiensis, Staphylococcus aureus, and Serratia marcescens. One-condition experiments. C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36501 | biostudies-arrayexpress

P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours

Project description:Transcriptional profiling of P. pacificus young adult worms exposed to pathogen Xenorhabdus nematophila for 4 hours versus age-matched worms exposed to control lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution of pathogen response by comparing this expression profile to that obtained by exposing the nematode C. elegans to the same pathogen. Other experiments which are a part of this study include expression profiling of C. elegans and P. pacificus on other pathogens including Bacillus thuringiensis DB27, Staphylococcus aureus, and Serratia marcescens. One-condition experiments. P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours. 4 biological replicates for each condition, including 2 dye-swaps.

2012-03-21 | E-GEOD-36523 | biostudies-arrayexpress

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Project description:Analysis of size selected RNA samples to identify sRNAs using wild-type and rpoS mutant strains. Four experiments, two from each of two strains using two protocols.

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C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours

2012-03-21 | GSE36501 | GEO

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