RNA Seq analysis of Mefs Isolated from STC1+/+ and STC1-/- mice

ABSTRACT: Evaluation of X-linked gene expression in MEFs isolated from STC1 wild type and knock out mice X chromosome inactivation (XCI) is initiated in cis by the Xist RNA, which coats the inactive X chromosome (Xi) from which it is produced. We performed a large-scale RNA interference screen to identify trans-acting XCI factors (XCIFs) that comprise regulators of cell signaling and transcription. We find that the XCIFs promote Xist expression and/or localization to the Xi. One of the XCIFs, STC1, is a glycoprotein found in both the cytoplasm and nucleus and whose function is poorly understood. A homozygous mouse knockout of STC1 has a defect in XCI but surprisingly is phenotypically normal. We performed transcriptome profiling (RNA-Seq) experiments to determine whether the expression levels of X-

ORGANISM(S): Mus musculus

SUBMITTER: Sanchita Bhatnagar 

PROVIDER: E-GEOD-47395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress