Staphylococcus aureus SH1000 compared to SH1000 M-NM-^TthyA

ABSTRACT: Staphylococcus aureus thymidine-dependent small-colony variants (TD-SCVs) are frequently isolated from patients with chronic S. aureus infections after long-term treatment with trimethoprim-sulfamethoxazole (TMP-SMX). In TD-SCVs, mutations of thymidylate synthase (thyA, TS), essential for DNA synthesis, occur. However, it has never been shown, that TMP-SMX is responsible for the induction and selection of TD-SCVs. Short-term exposure of TMP-SMX induced the TD-SCV phenotype morphologically as shown in transmission electron-microscopy and on the transcriptional level by qRT-PCR in wild-type S. aureus, while selection of TD-SCVs with thyA mutations occurred only rarely after long-term exposure. In reversion experiments with clinical TD-SCVs, all revertants revealed compensating mutations at t

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Jochen Seggewiss 

PROVIDER: E-GEOD-47406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress