Bisulfite-Seq profiling of wild-type Arabidopsis with two-component transgene silencing (T+S) system

ABSTRACT: In plants, RNA polymerase II (Pol II) transcription of inverted DNA repeats produces hairpin RNAs that are processed by several DICER-LIKE enzymes into siRNAs that are 21-24-nt in length. When targeted to transcriptional regulatory regions, the 24-nt size class can induce RNA-directed DNA methylation (RdDM) and transcriptional gene silencing (TGS). In a forward genetic screen to identify mutants defective in RdDM of a target enhancer leading to TGS of a downstream GFP reporter gene in Arabidopsis thaliana, we recovered a structurally mutated silencer locus, named SM-NM-^T35S, in which the 35S promoter driving transcription of an inverted repeat of target enhancer sequences had been specifically deleted. Although Pol II-dependent, hairpin-derived 21-24-nt siRNAs were no longer generated at

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Pao-Yang Chen 

PROVIDER: E-GEOD-47453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress