Role of Fft3 in nuclear organization [DamID-chip, ChIP-chip]

ABSTRACT: In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using genome-wide mapping and live cell imaging, we show that this role is linked to controlling nuclear organization of its targets. In fft3M-NM-^T cells, subtelomeres lose their association with the LEM domain protein Man1 at the nuclear periphery and move to the interior of the nucleus. Furthermore, genes in these domains are upregulated and active chromatin marks increase. Fft3 is also enriched at retrotransposon-derived long terminal repeat (LTR) elements at the borders of subtelomeres and at tRNA

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Babett Steglich 

PROVIDER: E-GEOD-47464 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress