High resolution view of bacteriophage lambda gene expression by ribosome profiling

ABSTRACT: Bacteriophage lambda is one of the most extensively studied organisms, and has been a primary model for understanding basic modes of genetic regulation. Here we examine the progress of lambda gene expression during phage development by ribosome profiling, and thereby provide a very high resolution view of lambda gene expression. The known genes are expressed in a predictable fashion, authenticating the analysis. But many previously unappreciated potential open reading frames become apparent in the expression analysis, revealing an unexpected complexity in the pattern of lambda gene function. We chose temperature induction of the classic cI857 repressor mutation in a lysogen of E. coli MG1655 in order to synchronize the lytic process, sampling the lysogen and control non-lysogen both bef

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Huifeng Jiang 

PROVIDER: E-GEOD-47509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress