Experimental characterization of the human non-sequence-specific nucleic acid interactome

ABSTRACT: The interactions between proteins and nucleic acids have a fundamental function in many biological processes well beyond nuclear gene transcription and include RNA homeostasis, protein translation and pathogen sensing for innate immunity. While our knowledge of the ensemble of proteins binding individual mRNAs in mammalian cells has greatly been augmented by recent surveys, no systematic study on the native proteins of human cells differentially engaging various types of nucleic acids in a non sequence-specific manner has been reported. We designed an experimental approach to cover the non sequence-specific RNA and DNA binding space broadly, including methylation, and test for its ability to interact with the human proteome. We used 25 rationally designed nucleic acid probes in an affinity

ORGANISM(S): Homo sapiens

SUBMITTER: Gerhard Duernberger 

PROVIDER: E-GEOD-47539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress