Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling analysis of S. pombe kinase deletin strains treated (60min) or untreated (0min) with nitrogen starvation in comparison with a common reference.

ABSTRACT: Kinase deletion strains treated with or without nitrogen starvation Background: Protein kinase-mediated signaling pathways play a pivotal role in regulation of stress responses. Nitrogen starvation (NS) induces sexual development when mating partner available or entry into a quiescent state (G0) in heterothallic background. However, it is unclear whether transcriptional profiles in response to NS allows distinguishing between kinase deletion strains defective in sexual development and/or G0-arrest. Results: In this study, we used DNA microarray to analyze transcriptional profiles in 68 protein kinase deletion strains prior to and after NS. Microarray analyses indicated that NS-induced transcriptional profiles of all kinase strains plus wild type are highly correlated with one another (i.e., PearsonÂ¡Â¯s correlation coefficient r > 0.7) except for that of pka1Â¦Â¤, which showed no high correlation with other 11 kinase strains. Significantly, 9 of 11 strains were defective in sexual development, suggesting that the NS-induced profile of pka1Â¦Â¤ serves as a signature with no high correlation with profiles of kinase strains defective in sexual development. Furthermore, we show that expression of the NS-induced genes is elevated in pka1Â¦Â¤ and cyr1Â¦Â¤ strains prior to NS. On the other hand, levels of the NS-induced genes expression in pka1Â¦Â¤, cyr1Â¦Â¤, sty1Â¦Â¤, and byr1Â¦Â¤ are significantly lower than that of wild type. Conclusions: Taken together, our analyses indicate that the cAMP-dependent Pka1-mediated signaling pathway play a major role in regulation of the NS-induced genes prior to and after NS. In addition, the ROS-activated kinase Sty1 and the pheromone-induced kinase Byr1 are also involved in up-regulation of NS-induced genes upon NS stress. 68 kinase deletion strains and other 5 deletion strains were treated with (60m) or without (0min) nitrogen starvation

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Jianhua Liu

PROVIDER: E-GEOD-47544 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Huarat2016 -Starvation-induced Ser/Thr protein kinase ArnS (Saci_1181) (Model 14) This model is described in the article: ArnS, a kinase involved in starvation-induced archaellum expression. Haurat MF, Figueiredo AS, Hoffmann L, Li L, Herr K, J Wilson A, Beeby M, Schaber J, Albers SV. Mol. Microbiol. 2016 Oct; : Abstract: Organisms have evolved motility organelles that allow them to move to favourable habitats. Cells integrate environmental stimuli into intracellular signals to motility machineries to direct this migration. Many motility organelles are complex surface appendages that have evolved a tight, hierarchical regulation of expression. In the crenearchaeon Sulfolobus acidocaldarius, biosynthesis of the archaellum is regulated by regulatory network proteins that control expression of archaellum components in a phosphorylation-dependent manner. A major trigger for archaellum expression is nutrient starvation, but although some components are known, the regulatory cascade triggered by starvation is poorly understood. In this work, the starvation-induced Ser/Thr protein kinase ArnS (Saci_1181) which is located proximally to the archaellum operon was identified. Deletion of arnS results in reduced motility, though the archaellum is properly assembled. Therefore, our experimental and modelling results indicate that ArnS plays an essential role in the precisely controlled expression of archaellum components during starvation-induced motility in Sulfolobus acidocaldarius. Furthermore they combined in vivo experiments and mathematical models to describe for the first time in archaea the dynamics of key regulators of archaellum expression. This model is hosted on BioModels Database and identified by: MODEL1607210000. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Transcriptional profiling analysis of S. pombe kinase deletin strains treated (60min) or untreated (0min) with nitrogen starvation in comparison with a common reference.

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