Microarray approach to investigate gene expression in human ovarian tissue after xenografting

ABSTRACT: Cryobanking and transplantation of ovarian tissue is a promising approach to restore fertility in cancer patients. However, ischemic stress following avascular ovarian cortex grafting is known to induce stromal tissue fibrosis and alteration in follicular development. The aim of the study is to analyse the impact of freezing-thawing and grafting procedures on gene expression in human ovarian tissue. Frozen-thawed ovarian tissue from 4 patients was xenografted for 7 days in nude mice and one non-grafted fragment was used as control. Immediately after recovery, grafts were processed for RNA extraction and histological analysis. Their expression profile was screened by whole-genome oligonucleotide array (n=4) and validated by reverse-transcriptase polymerase chain analysis (n=10). 84 of the t

ORGANISM(S): Homo sapiens

SUBMITTER: Jérôme Ambroise 

PROVIDER: E-GEOD-47555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress