Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Leukemia associated mutant Wilms' tumour gene 1 protein promotes expansion of human hematopoietic progenitor cells

ABSTRACT: Analysis of gene expression levels in hematopoietic progenitor cells retrovirally transduced with full-length WT1(+/-) or a WT1-mutant lacking zinc-finger, WT1(delZ) cultured in vitro for 14 days. The hypothesis tested in this study was that the WT1-mutant confers an increased proliferation rate after 14 days in culture and an erythroid phenotype. Results provide information of upregulation of some genes associated with cellcycle progression, upregulated genes connected with erythropoiesis and downmodulation of some genes associated with myeloid differentiation. Total RNA obtained from CD34+ progenitor cells in suspension cultures for 14 days.

ORGANISM(S): Homo sapiens

SUBMITTER: Karina Vidovic

PROVIDER: E-GEOD-47560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Identification of cell-type specific enhancers is important for understanding the regulation of programs controlling cellular development and differentiation. Enhancers are typically marked by the co-transcriptional activator protein p300 or by groups of cell-expressed transcription factors. We hypothesized that a unique set of enhancers regulates gene expression in human erythroid cells, a highly specialized cell type evolved to provide adequate amounts of oxygen throughout the body. Using chromatin immunoprecipitation followed by massively parallel sequencing, genome-wide maps of candidate enhancers were constructed for p300 and four transcription factors, GATA1, NF-E2, KLF1, and SCL, using primary human erythroid cells. These data were combined with gene expression analyses and candidate enhancers identified. Consistent with their predicted function as candidate enhancers, there was statistically significant enrichment of p300 and combinations of co-localizing erythroid transcription factors within 1-50 kb of the TSS of genes highly expressed in erythroid cells. Candidate enhancers were also enriched near genes with known erythroid cell function or erythroid cell phenotypes. Candidate enhancers exhibited only moderate conservation with mouse and minimal conservation with nonplacental vertebrates. Candidate enhancers were mapped to a data set of erythroid-associated, biologically relevant, SNPs from the GWAS catalog of the NHGRI. Fourteen candidate enhancers, representing 10 genetic loci, mapped to sites associated with biologically relevant erythroid traits. Fragments from these loci directed statistically significant expression in reporter gene assays. Identification of enhancers in human erythroid cells will allow a better understanding of erythroid cell development, differentiation, structure, and function, and provide insights into inherited and acquired hematologic disease. CD34+-selected stem and progenitor cells were expanded for three days in the absence of EPO, and total RNA was isolated from a portion of the cells. The cells were further cultured in the presence of EPO, and RNA was isolated after cells differentiated into R3/R4 nucleated erythroid cells. There were 3 replicates of CD34 cells and 9 replicates of R3/R4 erythroid cells.

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Leukemia associated mutant Wilms' tumour gene 1 protein promotes expansion of human hematopoietic progenitor cells

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