Regulation of Gene Expression by Connective Tissue Growth Factor (CTGF) in Mesenchymal Stromal Cells (MSCs)

ABSTRACT: mRNA from bone marrow-derived MSCs stably expressing CTGF-specific shRNA (or empty vector control) was analyzed for differential gene expression. Significant differences were found in cell proliferation-related genes, especially genes related to the M phase of the cell cycle, which were down-regulated in CTGF-knockdown-MSCs compared to control MSCs. Bone marrow-derived MSCs were stably transduced with lentivirus expressing CTGF-specific shRNA or an empty vector control. After antibiotic selection, total RNA was amplified and hybridized to Illumina HT12 version 3 human whole-genome arrays (Illumina, San Diego, CA).

ORGANISM(S): Homo sapiens

SUBMITTER: Wencai Ma 

PROVIDER: E-GEOD-47575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress