Species-specific factors mediate extensive heterogeneity of mRNA 3' ends in yeasts

ABSTRACT: Most eukaryotic genes express mRNAs with alternative polyadenylation sites at their 3’ ends. Here we show that polyadenylated 3’ termini in three yeast species (S. cerevisiae, K. lactis, D. hansenii) are remarkably heterogeneous. Instead of a few discrete 3’ ends, the average yeast gene has an “end zone”, a >200 bp window with >60 distinct poly(A) sites, the most utilized of which represents only 20% of the mRNA molecules. The pattern of polyadenylation within this zone varies across species, with D. hansenii possessing a higher focus on a single dominant point closer to the ORF terminus. Some polyadenylation occurs within mRNA coding regions with a strong bias towards the promoter. The polyadenylation pattern is determined by a highly degenerate sequence over a broad region and by a loc

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Zarmik Moqtaderi 

PROVIDER: E-GEOD-47661 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress