Derivation and maintenance of murine trophoblast stem cells under defined conditions

ABSTRACT: Trophoblast stem (TS) cells are in vitro equivalents to the precursor cells of the placenta. In mice, TS cells can be derived either from polar trophectoderm (TE) of blastocyst outgrowths or from postimplantation extraembryonic ectoderm (ExE), originating from polar TE. Since their first successful derivation in 1998 TS cells have been cultured in serum-rich medium in the presence of fibroblast growth factor 4 (FGF4), the cofactor heparin and embryonic fibroblast conditioned medium. Here, we developed and tested a simple medium consisting of ten chemically defined ingredients for routine culture of TS cells. The defined medium (TX medium) supports growth and self-renewal of TS cells grown on Matrigel-coated dishes over multiple (>20) passages. TS cells cultured under these conditions retai

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-47719 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress