TLR4/NF-κB-responsive microRNAs in a mouse model of skeletal muscle ischemia-reperfusion injury

ABSTRACT: TLR4/NF-κB signaling plays a central mediator in response to danger signals released in the muscle ischemia-reperfusion injury (IRI). This study was designed to profile TLR4/NF-κB-responsive microRNAs (miRNAs) in the skeletal muscles following IRI. Following 2 h of ischemia and subsequent reperfusion for indicated times (0 h, 4 h, 1 d, and 7 d) of the isolated thigh skeletal muscles based on femoral artery perfusion of C57BL/6, Tlr4–/–, and NF-κB–/–mice, the muscle specimens were analyzed with an miRNA array to detect the TLR4/NF-κB-responsive miRNAs. Male C57BL/6 mice (10–12 weeks of age, 22–35 g) were purchased from BioLasco (Taiwan). Tlr4–/– (C57BL/10ScNJ) and NF-κB–/– (B6.Cg-Nfkb1tm1Bal/J) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). All h

ORGANISM(S): Mus musculus

SUBMITTER: Ching-Hua Hsieh 

PROVIDER: E-GEOD-47730 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress