The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline

ABSTRACT: In a broad range of organisms, Piwi-interacting RNAs (piRNAs) have emerged as core components of a surveillance system that protects the genome by silencing transposable and repetitive elements. A vast proportion of piRNAs is produced from discrete genomic loci, termed piRNA clusters. The molecular mechanisms and the factors that govern the expression of these loci are largely unknown. We have preciously shown the Cutoff (Cuff), a protein with similarity to yeast Rai1, is a component of the piRNA pathway. In order to understand the function of the Cuff protein in piRNA production, we produced small RNA libraries from cn, bw (wt) and cuffwm25 mutant ovaties. The analysis of these libraries revealed that approximately 80% of the total piRNA population is depleted in the absence of a function

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Trudi Schupbach 

PROVIDER: E-GEOD-47738 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress