The regulatory role of ferric uptake regulator (Fur) during anaerobic respiration of Shewanella piezotolerans WP3
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ABSTRACT: Purpose: The goal is to study the regulatory role of ferric uptake regulator (Fur) during anaerobic respiration in Shewanella piezotolerans WP3 mRNA profiles of WP3 wild type (WP3) and the fur mutant (Fur) were generated by deep sequencing using Illumina HiSeq 2000.
Project description:Purpose: The goal is to study the regulatory role of ferric uptake regulator (Fur) during anaerobic respiration in Shewanella piezotolerans WP3
Project description:The cAMP binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD1 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The DNA binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD2 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 20MPa and 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 20M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment