Quantitative transcriptome analysis of a high-GSH producing Saccharomyces cerevisiae and the wild type based on the second generation sequencing.

ABSTRACT: The mutant Saccharomyces cerevisiae Y518 generated much more intracellular glutathione (GSH) than its counterpart dose. The RNA-seq based global transcriptome analysis was performed for exploring the potential mechanisms. Statistical analysis indicated that 1125 differentially expressed genes (fold-change>=2.0, FDR<=0.001) were up-regulated and 503 were down-regulated. There were 12 genes involved in glutathione metabolism. Of which GSH1, encodes gamma-glutamine cysteine synthetase, the rate-limiting enzyme in GSH biosynthesis process, was up-regulated. And MET17, encodes cysteine synthase A, an enzyme catalyzes the biosynthesis of one of the precursor amino acids L- cysteine, was also up-regulated. Besides, regulator SKN7 and several genes involved in oxidative stress response (GPX2, CTT1

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jiang Sun 

PROVIDER: E-GEOD-47787 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress