Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide expression profiling of HepG2 liver cells after berberine chloride treatment

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Ting-fang Lo

PROVIDER: E-GEOD-47822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

MicroRNA-21-3p, a berberine-induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth.

Lo Ting-Fang TF Tsai Wei-Chung WC Chen Shui-Tein ST

PloS one 20130930 9

Methionine adenosyltransferase (MAT) is the cellular enzyme that catalyzes the synthesis of S-adenosylmethionine (SAM), the principal biological methyl donor and a key regulator of hepatocyte proliferation, death and differentiation. Two genes, MAT1A and MAT2A, encode 2 distinct catalytic MAT isoforms. A third gene, MAT2B, encodes a MAT2A regulatory subunit. In hepatocellular carcinoma (HCC), MAT1A downregulation and MAT2A upregulation occur, known as the MAT1A:MAT2A switch. The switch is accomp ...[more]

PMID: 24098708

Similar Datasets

MicroRNA expression profiling of HepG2 liver cells after berberine chloride treatment

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects including anti-cancer effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in HCC. Here, we used miRNA microarray to analyze microRNA expression profiles of HepG2 human hepatoma cell line after berberine chloride treatment or 0.08% DMSO as control. Comparing miRNA profiles of 40 M-BM-5M-BM--M berberine-treated HepG2 human hepatoma cell line to those of control cells sampled after 2 and 4 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 M-BM-5M-BM--M berberine chloride or 0.08% DMSO as control.

2013-10-17 | E-GEOD-47821 | biostudies-arrayexpress

Genome-wide analysis of berberine chloride-responsive gene expression in HepG2 liver cell line

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Here, we used gene expression microarray to analyze gene expression profiles of HepG2 human hepatoma cell line after berberine chloride treatment or 0.08% DMSO as control. Comparing gene expression profiles of 40 M-BM-5M-BM--M berberine-treated HepG2 human hepatoma cell line to those of control cells sampled after 4 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 M-BM-5M-BM--M berberine chloride or 0.08% DMSO as control.

2013-10-02 | E-GEOD-47786 | biostudies-arrayexpress

MicroRNA and gene expression profiling of primary human hepatocytes after berberine chloride treatment

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-12-31 | E-GEOD-53416 | biostudies-arrayexpress

MicroRNA expression profiling of primary human hepatocytes after berberine chloride treatment

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in liver. Here, we used miRNA microarray to analyze microRNA expression profiles of primary human hepatocytes after berberine chloride treatment or 0.08% DMSO as control. Comparing miRNA profiles of 40 ïM berberine-treated primary human hepatocytes to those of control cells sampled after 2 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 ïM berberine chloride or 0.08% DMSO as control.

2015-12-31 | E-GEOD-53413 | biostudies-arrayexpress

Gene expression profiling of primary human hepatocytes after berberine chloride treatment

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in liver. Here, we used miRNA microarray to analyze microRNA expression profiles of primary human hepatocytes after berberine chloride treatment or 0.08% DMSO as control. Comparing gene expression profiles of 40 ï?M berberine-treated primary human hepatocytes to those of control cells sampled after 2, 4, or 8 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 ï?M berberine chloride or 0.08% DMSO as control.

2015-12-31 | E-GEOD-53415 | biostudies-arrayexpress

The differentially expressed genes of Zucker Diabetic Fatty Rats treated with Berberine against diabetes mellitus

Project description:The anti-diabetes activity of Rhizoma Coptidis was documented in the book âNotes of Elite Physiciansâ written by Hongjing Tao 1500 years ago. Berberine (BBR) is the major isoquinoline alkaloid constituent of the Chinese herb Rhizoma Coptidis, which has the beneficial characteristic of regulating glucose and lipid metabolism and has been extensively used in the treatment of obesity, diabetes and hypercholesterolemia. However, the effect of berberine on specific diabetes treatment targets is unknown. This study was conducted to elucidate the genome-wide gene expression profile in Zucker Diabetic Fatty Rats liver tissues in response to Berberine treatment.

2016-11-30 | E-MTAB-5178 | biostudies-arrayexpress

Gene expression in response to fluconazole vs. the combination of fluconazole and berberine

Project description:Transcriptional profiling of Candida albicans comparing fluconazole treated cells with fluconazole- and berberine-treated cells, as well untreated cells with berberine treated cells Three different clinical FLC-resistant strains (0304103, 01010 and 632) were selected to carry out the expression profile microarray. Two-condition experiment, fluconazole-treated vs. fluconazole- and berberine-treated cells, and untreated cells vs. berberine-treated cells. Biological replicates: 3 control, 3 transfected, independently grown and harvested. One replicate per array.

2013-11-14 | E-GEOD-43662 | biostudies-arrayexpress

Identification of a novel biomarker serum miRNA from the patients with colorectal cancer (CRC).

Project description:Serum microRNAs (miRNAs) have been shown to have a potential for cancer diagnosis lately. The main objective of this study is to identify a novel biomarker serum miRNA from the patients with colorectal cancer (CRC). Microarray analysis of miRNA expression was performed using paired pre- and post- operative serum from 10 CRC patients. Two miRNAs (let-7a, miR-199a-3p) decreased significantly in the post-operative serum when compared to pre-operative serum (P=0.015 and 0.029, respectively). Microarrays were performed for paired pre- and post- operative serum from 10 CRC patients.

2014-10-02 | E-GEOD-55139 | biostudies-arrayexpress

MicroRNA expressions in human hepatocarcinoma cells in response to HBV X protein expression

Project description:MicroRNA expression profiling of human Hep3B and HepG2 HCC cells comparing parental cancer cells with stable HBx-transfectants In this study,two pairs of control and HBx-overexpressing HCC cells were used to acquire expression profiles of a total of microRNA. miRNA microarray analysis was performed with mirVana miRNA Bioarrays V1 (Ambion) according to the manufacturerM-bM-^@M-^Ys instructions

2014-04-02 | E-GEOD-56424 | biostudies-arrayexpress

Expression Profiles of Primary Mouse Hepatocytes treated with Cyclosporin A and solvent control [miRNA]

Project description:The microRNA changes induced in Primary Mouse Hepatocytes of C57Bl6-mice by Cyclosporin A after treatment for 24h and 48h The study investigated differential microRNA expression in Primary Mouse Hepatocytes following 24 and 48 hours of exposure to Cyclosporin A and solvent. Three biological replicates per compound/six per solvent. In total 24 arrays .

2014-07-24 | E-GEOD-55880 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data