Transcriptional analysis of select CfMNPV genes and some antisense transcripts by an oligonucleotide microarray

ABSTRACT: The temporal expression of the 23 CfMNPV genes representing all four temporal classes including its 7 unique genes were determined by a modified oligonucleotide-based two-channel DNA microarray. Transcription of the non-coding (antisense) strands of some of the CfMNPV select genes including the polyhedrin gene was also detected by the array analysis. The expression of four host genes varied several fold throughout virus infection. The microarray chip contained oligonucleotide probes for 23 CfMNPV ORFs and their complements. We first developed a novel normalization protocol using Cy5-labeled CfMNPV viral genomic DNA (vgDNA) as equimolar reference standards for each probe in order to overcome the inherent variability problem of the traditional microarray normalization procedures including us

ORGANISM(S): Choristoneura fumiferana MNPV

SUBMITTER: Mehrnoosh Barari 

PROVIDER: E-GEOD-4783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress