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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Jurkat CF4+ T cells transfected with simian immunodeficiency virus Nef cells leads to a molecular profile of viral persistence and immune evasion.

ABSTRACT: Jurkat T cell line was transfected with SIV Nef controlled by an inducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Experiment Overall Design: Experimental conditions: 24 hr SIV Nef expression at physiological concentrations in T cells. Experiment Overall Design: Controls: Non-transfected. 24 hr.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael George

PROVIDER: E-GEOD-4785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of simian immunodeficiency virus Nef protein in CD4+ T cells leads to a molecular profile of viral persistence and immune evasion.

Ndolo Thomas T George Michael M Nguyen Hau H Dandekar Satya S

Virology 20060720 2

The Nef protein of human immunodeficiency virus and simian immunodeficiency virus is expressed early in infection and plays an important role in disease progression in vivo. In addition, Nef has been shown to modulate cellular functions. To decipher Nef-mediated changes in gene expression, we utilized DNA microarray analysis to elucidate changes in gene expression in a Jurkat CD4+ T-cell line stably expressing SIV-Nef protein under the control of an inducible promoter. Our results showed that ge ...[more]

PMID: 16857233

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Nef transfection of Jurkat T cells

Project description:Jurkat T cell line was transfected with SIV Nef controlled by an unducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Keywords: SIV Nef, Jurkat, pronasterone A

2006-07-25 | GSE4785 | GEO

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