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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Murine E9.5 embryo PSM and newly formed somites: Paraxis-/- vs WT

ABSTRACT: Transcriptional profiling of E9.5 mouse embryo tissue from the presomitic mesoderm (PSM) and somites I-IV. Tissue from embryos lacking a functional Paraxis gene (Paraxis-/-) was compared to identical tissue from E9.5 Wild Type embryos. The goal was to identify genes that had become deregulated in the absence of the transcription factor, Paraxis. Two-condition experiment: WT vs Paraxis-/- tissue. Biological replicates: 3 pools of 5 WT samples, 3 pools of 5 Paraxis-/- samples. Technical replicates: 3 dye swaps.

ORGANISM(S): Mus musculus

SUBMITTER: Megan Rowton

PROVIDER: E-GEOD-47905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.

Rowton Megan M Ramos Pilar P Anderson Douglas M DM Rhee Jerry M JM Cunliffe Heather E HE Rawls Alan A

Developmental dynamics : an official publication of the American Association of Anatomists 20130930 11

<h4>Background</h4>Dynamic alterations in cell shape, migration, and adhesion play a central role in tissue morphogenesis during embryonic development and congenital disease. The mesenchymal-to-epithelial transition that occurs during vertebrate somitogenesis is required for proper patterning of the axial musculoskeletal system. Somitic MET is initiated in the presomitic mesoderm by PARAXIS-dependent changes in cell adhesion, cell polarity, and the composition of the extracellular matrix. Howeve ...[more]

PMID: 24038871

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Murine E9.5 embryo PSM and newly formed somites: Paraxis-/- vs WT

Project description:Transcriptional profiling of E9.5 mouse embryo tissue from the presomitic mesoderm (PSM) and somites I-IV. Tissue from embryos lacking a functional Paraxis gene (Paraxis-/-) was compared to identical tissue from E9.5 Wild Type embryos. The goal was to identify genes that had become deregulated in the absence of the transcription factor, Paraxis.

2013-12-05 | GSE47905 | GEO

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Project description: Not available

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2023-01-24 | E-MTAB-12539 | biostudies-arrayexpress

RNA-seq of mouse individual epithelial somites through development

Project description:Somites are transient embryonic structures consisting of hundreds of cells that bud off from the anterior tip of the presomitic mesoderm on each side of the neural tube. They give rise to the vertebrae and associated skeletal muscles, nerves, and blood vessels. To characterise the transcriptional changes that orchestrate mouse somitogenesis, we have generated coupled RNA-seq and ATAC-seq profiles of individual somites, across embryonic development. We collected the three most posterior pairs of somites, which correspond to those most recently segmented, from embryos containing 8, 18, 21, 25, 27 and 35 pairs of somites. The three somites are labelled I, II and III from the most posterior to the most anterior. From each pair, one somite was used for RNA-seq and the other one for ATAC-seq.

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