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Murine E9.5 embryo PSM and newly formed somites: Paraxis-/- vs WT

ABSTRACT: Transcriptional profiling of E9.5 mouse embryo tissue from the presomitic mesoderm (PSM) and somites I-IV. Tissue from embryos lacking a functional Paraxis gene (Paraxis-/-) was compared to identical tissue from E9.5 Wild Type embryos. The goal was to identify genes that had become deregulated in the absence of the transcription factor, Paraxis. Two-condition experiment: WT vs Paraxis-/- tissue. Biological replicates: 3 pools of 5 WT samples, 3 pools of 5 Paraxis-/- samples. Technical replicates: 3 dye swaps.

ORGANISM(S): Mus musculus

SUBMITTER: Megan Rowton

PROVIDER: E-GEOD-47905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of mesenchymal-to-epithelial transition by PARAXIS during somitogenesis.

Rowton Megan M Ramos Pilar P Anderson Douglas M DM Rhee Jerry M JM Cunliffe Heather E HE Rawls Alan A

Developmental dynamics : an official publication of the American Association of Anatomists 20130930 11

<h4>Background</h4>Dynamic alterations in cell shape, migration, and adhesion play a central role in tissue morphogenesis during embryonic development and congenital disease. The mesenchymal-to-epithelial transition that occurs during vertebrate somitogenesis is required for proper patterning of the axial musculoskeletal system. Somitic MET is initiated in the presomitic mesoderm by PARAXIS-dependent changes in cell adhesion, cell polarity, and the composition of the extracellular matrix. Howeve ...[more]

PMID: 24038871

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